Journal: Cancer Biology & Medicine

Article Title: FDX1 expression promotes DSF/Cu-induced cuproptosis in gastric cancer cells

doi: 10.20892/j.issn.2095-3941.2025.0323

Figure Lengend Snippet: Effect of WDR43 knockdown on GC cells. (A) Western blot detection of WDR43 protein expression in GC cells with WDR43 knockdown ( n = 3). (B) Statistical analysis of western blot. (C) CCK-8 detected the proliferation of non-treated GC (CON) and GC cells (siWDR43) after knocking down WDR43 ( n = 3). (D) Invasion assay to detect the invasion ability of cells ( n = 3) (scale bar, 100 μm). (E) Statistical analysis of the results of the invasion experiment ( n = 3). (F) Scratch healing assay to detect cell migration ( n = 3) (scale bar, 500 μm). (G) Statistical analysis of the results of scratch healing experiments ( n = 3). (H) Cell cycle was measured by flow cytometry ( n = 3). (I) Statistical analysis of cell cycle test results ( n = 3). (J) Western blot analysis was performed to detect the levels of CDK2 , cyclinD-1 , and DLAT gene expression in GC cells with FDX1 knockout ( n = 3). (K) Statistical analysis of western blot. Data are expressed as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001, indicating statistically significant differences.

Article Snippet: The subsequent process included separating the proteins through SDS-PAGE, transferring the proteins onto a PVDF membrane, and combining the membrane with primary antibodies against FDX1, DLAT, WDR43, cyclin dependent-kinase 2 (CDK2), cyclinD-1, and β-actin (Proteintech, Wuhan, China).

Techniques: Knockdown, Western Blot, Expressing, Analysis, CCK-8 Assay, Invasion Assay, Migration, Flow Cytometry, Gene Expression, Knock-Out

Journal: Journal of Translational Medicine

Article Title: Toosendanin suppresses acute myeloid leukemia by targeting DDX5/c-Myc axis to inhibit protein synthesis

doi: 10.1186/s12967-026-08339-z

Figure Lengend Snippet: TSN inhibits AML cell proliferation and induces cell cycle arrest. ( A ) Viability of AML cell lines (MV4-11, MOLM-13, THP-1, and HL-60) treated with TSN for 48 h, as evaluated by CCK-8 assay. Calculated half-maximal inhibitory concentrations (IC₅₀) values are shown. (B, C) Cell cycle distribution of MOLM-13 and MV4-11 cells treated with TSN or DMSO for 48 h, as analyzed by EdU incorporation and flow cytometry. ( B ) Representative flow cytometry plots. ( C ) Quantitative analysis of cells in the G0/G1, S, and G2/M phases ( n = 3). ( D ) Colony-forming capacity of MOLM-13 and MV4-11 cells cultured in methylcellulose medium containing TSN for 8 days. ( E , F ) Western blot analysis of cell cycle regulators CDK1 (34 kDa) and CDK2 (33 kDa) in MOLM-13 and MV4-11 cells after 48 h of TSN treatment ( n = 3). GAPDH (36 kDa) served as the loading control. Data are expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: Primary antibodies used for western blot analysis included: β-actin (AC004, ABclonal, Wuhan, China; 1:1000), glyceraldehyde-3-phosphate dehydrogenase (GAPDH, AF1186, Beyotime; 1:1000), β-Tubulin (10068-1-AP, Proteintech, Wuhan, China; 1:1000), CDK1 (A22347, ABclonal; 1:1000), CDK2 (A0294, ABclonal; 1:1000), Caspase-3/Cleaved Caspase-3 (ET1608-64, HUABIO, Hangzhou, China; 1:1000), PARP/Cleaved PARP ( T40050 , Abmart, Shanghai, China; 1:1000), c-Myc ( T55150 , Abmart; 1:1000), and DDX5 (ET1705-32, HUABIO; 1:1000).

Techniques: CCK-8 Assay, Flow Cytometry, Cell Culture, Western Blot, Control